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In vivo transfer of pAMβ1 from Lactobacillus reuteri to Enterococcus faecalis
Author(s) -
Morelli L.,
Sarra P. G.,
Bottazzi V.
Publication year - 1988
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1988.tb01905.x
Subject(s) - lactobacillus reuteri , enterococcus faecalis , microbiology and biotechnology , in vivo , plasmid , transferability , lactobacillus , antibiotics , bacteria , strain (injury) , biology , enterococcus , antibiotic resistance , staphylococcus aureus , dna , genetics , statistics , mathematics , logit , anatomy
Trials were conducted to determine the in vivo transferability of plasmid‐mediated antibiotic resistance between two strains of enteric Gram‐positive bacteria. Germfree mice were associated with the donor Lactobacillus reuteri DSM 20016 strain, carrying the broad host range pAMβ1 plasmid, and with the Enterococcus faecalis JH2SS recipient strain. Analysis of faecal content of associated mice demonstrated that the in vivo transfer of this plasmid did occur and that frequencies of conjugation were affected by the presence of subtherapeutic levels of antibiotic in the diet.