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Growth energetics of Clostridium sporogenes NCIB 8053: modulation by CO 2
Author(s) -
Dixon N.M.,
Lovitt R.W.,
Morris J.G.,
Kell† D.B.
Publication year - 1988
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1988.tb01500.x
Subject(s) - carbon dioxide , chemostat , partial pressure , clostridium sporogenes , yield (engineering) , energetics , axenic , amino acid , chemistry , biochemistry , clostridium , biology , oxygen , biophysics , materials science , ecology , organic chemistry , bacteria , metallurgy , genetics
The effects of the partial pressure of carbon dioxide on the growth energetics of Clostridium sporogenes NCIB 8053 grown in chemostat culture were investigated in defined minimal media. Both the ‘maintenance’ requirements and the growth yield coefficients were dependent upon the partial pressure of carbon dioxide in otherwise glucose‐limited cultures. Since growth yield coefficients decreased along with the apparent ‘maintenance’ requirements in essential amino acid/fatty acid medium when the partial pressure of carbon dioxide was increased above 0.5 atm, the occurrence of some type of metabolic uncoupling seemed likely. By contrast, when the organism was grown in amino acid complete medium both the maintenance requirements and the growth yield coefficients were increased when the partial pressure of carbon dioxide was raised above 0.5 atm partial pressure of carbon dioxide, suggesting an increased efficiency of growth. A futile cycle involving carbon dioxide is proposed as a factor contributing to the variable extent of free energy dissipation within this organism.