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Associative cellulolysis and N 2 fixation by co‐cultures of Trichoderma harzianum and Clostridium butyricum : the effects of ammonium‐N on these processes
Author(s) -
Veal D. V.,
Lynch J. M.
Publication year - 1987
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1987.tb04943.x
Subject(s) - clostridium butyricum , trichoderma harzianum , diazotroph , cellulose , microbiology and biotechnology , nitrogen fixation , food science , biology , ammonium , botany , biochemistry , chemistry , fermentation , bacteria , organic chemistry , biological pest control , genetics
Mixed cultures of the cellulolytic fungus Trichoderma harzianum with the anaerobic diazotroph Clostridium butyricum were shown to co‐operatively degrade cellulose and utilize the degradation products for N 2 fixation. Cellulose degradation and N 2 fixation were stimulated by small (0.1 mg/ml) additions of (NH 4 ) 2 SO 4 . The (NH 42 SO 4 stimulates cellulolysis thereby increasing the supply of cellulose degradation products to the diazotroph. In aerobic environments the anaerobe depends on the respiration of the aerobe to create anaerobic microsites. The N source increased O 2 uptake by the fungus increasing the number of sites suitable for the development of the anaerobe. Stimulation in the growth of T. harzianum by (NH 42 SO 4 resulted in increased growth and N 2 fixation by Cl. butyricum.