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A low‐cost antifoam additive for agar‐based culture media
Author(s) -
Blenkharn J. I.,
Wood Sharon
Publication year - 1987
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1987.tb04870.x
Subject(s) - defoamer , agar , emulsion , silicone , glycerol , food science , microbiology and biotechnology , chemistry , bacteria , biology , biochemistry , organic chemistry , physics , dispersant , optics , genetics , dispersion (optics)
A highly effective, low‐cost silicone‐based antifoam emulsion is described which, at a final concentration of 100 ppm, prevents bubble formation during the preparation and dispensing of agar media. The compound is heat‐stable, has a long shelf‐life and offers considerable savings in cost by reduction in wastage of time and materials. It has no demonstrable deleterious effect on the growth of a wide range of pathogenic and commensal bacteria, or on antibiotic disc susceptibility testing, when examined using a range of different media.