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The effect of incubation time and temperature on growth of Escherichia coli on gradient plates containing sodium chloride and sodium nitrite
Author(s) -
McClure P.J.,
Roberts T.A.
Publication year - 1987
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1987.tb04861.x
Subject(s) - sodium nitrite , incubation , sodium , chemistry , preservative , nitrite , escherichia coli , staining , eosin , chromatography , tetrazolium chloride , biochemistry , food science , nuclear chemistry , biology , organic chemistry , nitrate , medicine , ischemia , cardiology , genetics , gene
Two‐dimensional gradient plates are a convenient way of screening antimicrobial effects of preservative factors acting in combination across a broad range of physical and chemical conditions. We report the effects of sodium chloride, sodium nitrite and incubation temperature on the growth of Escherichia coli by staining, laser densitometry and computer graphics. Staining not only more easily distinguished the growth area but also gave an indication of the viability of cells present. 2‐( p ‐iodophenyl)‐3‐( p ‐nitrophenyl)‐5‐phenyl tetrazolium chloride was the more useful of the two stains used. Inhibitory concentrations of sodium chloride decreased with reduced incubation temperature. The response of E. coli to combinations of salt and nitrite on gradient plates was very similar to its response in liquid medium.