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Use of the direct epifluorescent filter technique for the enumeration of bacterial spores
Author(s) -
Kelly Alison F.,
Kroll R. G.
Publication year - 1987
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1987.tb02725.x
Subject(s) - spore , staining , orange (colour) , plate count , endospore , enumeration , microbiology and biotechnology , biology , viable count , germination , incubation , yeast , food science , bacteria , chemistry , botany , fermentation , biochemistry , mathematics , genetics , combinatorics
Heat treatment at 80°C for 10 min effectively destroyed all vegetative cells (except for Gram‐positive cocci) and made easier the counting of bacterial spores, which stained orange, green or rarely transparent/black with a dull green halo, in the direct epifluorescent filter technique. The numbers of both orange‐ or green‐staining spores were lower than the plate count. A variety of physiological conditions were used to investigate the relationship of the different staining patterns with germination status. It was concluded that orange‐staining spores had germinated and their number agreed with the plate count after incubation in yeast glucose broth at 30°C for 4 h. This observation was unreliable, however, but it was found that a total spore count in the DEFT gave a good agreement with the plate count.