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Effects of p CO 2 on the growth and metabolism of Clostridium sporogenes NCIB 8053 in defined media
Author(s) -
Dixon N. M.,
Lovitt R. W.,
Kell D. B.,
Morris J. G.
Publication year - 1987
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1987.tb02700.x
Subject(s) - clostridium sporogenes , fermentation , carbon dioxide , metabolism , clostridium , partial pressure , amino acid , chemistry , biochemistry , bacterial growth , food science , stereochemistry , biology , bacteria , organic chemistry , genetics , oxygen
The effects of the partial pressure of carbon dioxide ( p CO 2 ) on the growth rate of Clostridium sporogenes NCIB 8053 in batch culture were investigated in defined minimal media. Depending upon the growth medium, CO 2 was stimulatory or inhibitory to growth. The absolute CO 2 requirement for growth displayed by Cl. sporogenes in certain media was shown to be due to the involvement of CO 2 in the synthesis of branched‐chain amino acids. High concentrations of CO 2 were growth inhibitory except in complex media. In media where an optimal p CO 2 was observed it was approximately 0˙45 atm. The pattern of fermentation end‐products displayed by this organism was also modified by the p CO 2 . The equilibria of the different species of ‘CO 2 ’ present in microbiological media are described. Finally, the metabolic control theory of Kacser, Burns, Heinrich and Rapoport has been applied to the results obtained, to provide a quantitative description of the effects of p CO 2 on the growth rate of Cl. sporogenes.