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Comparison of most probable number and pour plate procedures for isolation and enumeration of sulphite‐reducing Clostridium spores and Group D faecal streptococci from oysters
Author(s) -
Easterbrook T. J.,
West P. A.
Publication year - 1987
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1987.tb02670.x
Subject(s) - enumeration , spore , isolation (microbiology) , clostridium , microbiology and biotechnology , most probable number , clostridia , biology , feces , group (periodic table) , chemistry , bacteria , mathematics , combinatorics , genetics , organic chemistry
A comparative study of methods to enumerate sulphite‐reducing Clostridium spores and Group D faecal streptococci in oysters demonstrated that pour plate solid agar techniques gave higher counts than liquid broth most probable number procedures. Reinforced clostridial broth with supplements to detect sulphite reduction was compared with pour plates of egg yolk‐free tryptose sulphite cycloserine agar incubated at 37°C for 24 h. Azide dextrose broth was compared with pour plates using Slanetz and Bartley (SB) agar or KF‐streptococcus agar at 37°C. Most probable number procedures used for both groups of organisms gave excessive numbers of improbable tube combinations. For enumeration of Group D faecal streptococci, a pour plate technique using SB agar incubated at 37°C for 48 h is recommended.