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Ampicillin‐dextrin agar medium for the enumeration of Aeromonas species in water by membrane filtration
Author(s) -
Havelaar A. H.,
During M.,
Versteegh J. F. M.
Publication year - 1987
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1987.tb02410.x
Subject(s) - agar , aeromonas , filtration (mathematics) , chromatography , ampicillin , incubation , microbiology and biotechnology , isolation (microbiology) , chemistry , food science , bacteria , dextrin , biology , biochemistry , antibiotics , statistics , genetics , mathematics , starch
Published selective media were evaluated for the isolation of Aeromonas spp. from environmental samples by membrane filtration. Satisfactory recoveries were obtained only with mA agar (Rippey & Cabelli) and dextrin‐fuchsin‐sulphite agar (Schubert), but neither was sufficiently selective. The positive aspects of these two media were combined in a new medium, ampicillin‐dextrin agar. Recovery from pure cultures and environmental samples was optimal at an ampicillin concentration of 10 mg/l and incubation for 24 h at 30°C under aerobic conditions, and specificity was high (i.e. confirmation rate usually <90%, no false negative colonies encountered). The medium can also be used for isolation of Aeromonas spp. from sea water provided that the vibriostatic agent 0/129 is added at 50 mg/1.