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Purification and properties of extracellular polysaccharide (EPS) antigens produced by different mould species
Author(s) -
Notermans S.,
Wieten G.,
Engel H. W. B.,
Rombouts F. M.,
Hoogerhout P.,
van Boom J. H.
Publication year - 1987
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1987.tb02394.x
Subject(s) - cladosporium cladosporioides , polysaccharide , rhamnose , penicillium , galactose , sepharose , biology , microbiology and biotechnology , penicillium digitatum , biochemistry , monosaccharide , chemistry , botany , enzyme , antifungal
Extracellular polysaccharide (EPS) antigens produced by different mould species were purified and partially characterized. Purification included (NH 4 ) 2 SO 4 treatment, Sepharose CL‐4B column chromatography and Con A‐sepharose chromatography. The EPS of Penicillium digitatum, Mucor racemosus and Cladosporium cladosporioides showed high antigenic capacities. Immunologically the EPS were partially genus‐specific, but cross‐reactivity was observed. The EPS antigens produced by species of Penicillium, Aspergillus repens and Geotrichum candidum lost their immunological activity upon heating (100C) at pH 18, while the EPS antigen of M. racemosus, Rhizopus oligosporus and C. cladosporioides were stable under the same conditions. The dominant monosaccharides present in the EPS antigen were mannose, galactose and glucose. The EPS obtained from cultures of M. racemosus and R. oligosporus also contained rhamnose. In the EPS produced by Penicillium spp. and A. repens the galactose residues were determined to be immunodominant.