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Water relations of solute accumulation in Pseudomonas fluorescens
Author(s) -
Prior B. A.,
Kenyon C. P.,
van der Veen Michelle,
Mildenhall J. P.
Publication year - 1987
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1987.tb02389.x
Subject(s) - pseudomonas fluorescens , water activity , sorbitol , chemistry , glutamic acid , food science , intracellular , biochemistry , amino acid , bacteria , biology , water content , genetics , geotechnical engineering , engineering
When Pseudomonas fluorescens was grown in a glucose salts medium adjusted with NaCl to a water activity (a w ) value of 0.980, the intracellular glutamic acid concentration increased 23‐fold and comprised 90% of the total amino acid pool. This increase was not observed when the a w of the medium was reduced to 0.980 with sorbitol. Sorbitol was taken up rapidly over a 30 min period and accumulated intracellularly to a level approximately two‐fold greater than the concentration in the growth medium. In continuous culture, the specific rate of glutamic acid production and glucose uptake was greater at 0.980 (NaCl) than at 0.997 a w . The maintenance coefficients for glucose uptake were similar at both a w values but were 2.4‐fold greater for glutamic acid production at 0.980 (NaCl) than at 0.997 a w .