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Germination of spores of Bacillus subtilis var. niger following exposure to gaseous ethylene oxide
Author(s) -
Dadd A. H.,
Rumbelow Janet E.
Publication year - 1986
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1986.tb05088.x
Subject(s) - germination , spore , bacillus subtilis , valine , ethylene oxide , asparagine , alanine , chemistry , isoleucine , food science , amino acid , horticulture , microbiology and biotechnology , leucine , biology , biochemistry , bacteria , organic chemistry , genetics , copolymer , polymer
Spores of Bacillus subtilis var. niger exposed to a lethal dose of ethylene oxide (ETO) germinated freely under a variety of nutritional conditions. Outgrowth, however, did not occur. Good germinants for ETO‐exposed spores were (in order of decreasing effectiveness) alanine, valine, cysteine, isoleucine and histidine. Asparagine, while a germinant for unexposed spores, did not allow the germination of ETO‐exposed spores. Mixtures of amino acids were no more effective than alanine alone. Exposure to ETO lowered both the rate and amount of germination but the effect was much less than that on viability. A linear relationship was obtained by plotting germination in glucose alanine against survivors on a logarithmic scale. This relationship did not occur when valine was the germinant.