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Amylase from Lactobacillus cellobiosus D‐39 isolated from vegetable wastes: Purification and characterization
Author(s) -
Sen Sribir,
Chakrabarty S. L.
Publication year - 1986
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1986.tb05087.x
Subject(s) - lactobacillus , amylase , chromatography , chemistry , cellulose , precipitation , lactobacillaceae , bacteria , enzyme , food science , biochemistry , biology , fermentation , physics , meteorology , genetics
An organism producing α‐amylase and identified as Lactobacillus cellobiosus D‐39 was recently isolated from vegetable wastes. Its amylase was purified by (NH 4 ) 2 SO 4 precipitation and DEAE cellulose column chromatography and was obtained in crystalline form. It was fairly stable at a broadly neutral pH range and maximally active at 50°C. The molecular weight was 22 500 daltons as determined by SDS gel electrophoresis.