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Selection of pH buffers for use in conductimetric microbiological assays
Author(s) -
Owens J. D.,
WacherViveros M. C.
Publication year - 1986
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1986.tb05084.x
Subject(s) - hydroxymethyl , tris , chemistry , histidine , phosphate , phosphate buffered saline , buffer (optical fiber) , chromatography , fermentation , conductivity , biochemistry , amino acid , organic chemistry , telecommunications , computer science
Many metabolic activities of micro‐organisms lead to changes in the pH value of cultures and consequently pH buffer compounds are potentially a major source of conductivity changes in cultures. To maximize changes in conductivity associated with microbial growth the pH buffer‐associated changes should occur in a direction that reinforces those due to other metabolic activities. In agreement with this, studies with Escherichia coli showed that fermentation of glucose and aerobic growth on L‐alanine yielded greater changes in the conductivity of media containing Tris(hydroxymethyl)aminomethane or L‐histidine buffers than in a medium containing phosphate buffer, whereas aerobic growth on glucose or succinate yielded greater changes with phosphate buffer than with Tris(hydroxymethyl)aminomethane or L‐histidine buffers. Criteria for the selection of appropriate pH buffer compounds are presented.