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A note on the effect of nitrogen source on growth of and solvent production by Clostridium acetobutylicum
Author(s) -
Welsh F. W.,
Williams R. E.,
Veliky I. A.
Publication year - 1986
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1986.tb04305.x
Subject(s) - clostridium acetobutylicum , ammonium , chemistry , subculture (biology) , solvent , fermentation , asparagine , food science , nitrogen , ammonium acetate , ammonium sulfate , amino acid , biochemistry , organic chemistry , ethanol , botany , biology , butanol , high performance liquid chromatography
A number of mono‐ and dicarboxylic acid amino acids, amides and inorganic ammonium sources were tested to determine their effect on the growth of and solvent production by Clostridium acetobutylicum ATCC 824. Optimum cell growth (A 650 = 0.96) and solvent production (total solvents = 10.85 g/l) were obtained with the medium containing glutamic acid as the ammonium source. Similar results were obtained through three successive serial subcultures although changes in the fermentation were noted with the third subculture. Cell growth and solvent production fell after the second subculture when ammonium acetate was used as the ammonium source. Adaptation was shown to occur in a medium which contained asparagine.