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Diseased leaf lyophilization: a method for long‐term prevention of loss of virulence in phytopathogenic bacteria
Author(s) -
Bashan Y.,
Okon Y.
Publication year - 1986
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1986.tb04271.x
Subject(s) - xanthomonas campestris , virulence , pathogenicity , pathogen , biology , pseudomonas syringae , bacteria , microbiology and biotechnology , host (biology) , pathogenic bacteria , xanthomonas , pseudomonas , ecology , biochemistry , genetics , gene
A technique for long‐term preservation of phytopathogenic bacteria is described. It is based on selective multiplication of the bacterial pathogen in host tissue, disinfection of leaf surfaces to reduce contamination, lyophilization of leaves, and storage under dry conditions at ‐ 80°C. With this technique, the pathogenicity of large numbers of the pathogens Pseudomonas syringae pv. tomato and Xanthomonas campestris pv. vesicatoria was maintained for 4 years.