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Induced release of Bacillus spores from sporangia by sodium sulphate
Author(s) -
Burnett A. M.,
Jr. J. W. Ezzell,
Singh J.,
Jr. G. F. Zipperle,
Doyle R. J.
Publication year - 1986
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1986.tb01741.x
Subject(s) - sporangium , spore , bacillus subtilis , bacillus cereus , lysis , microbiology and biotechnology , sodium , cereus , incubation , endospore , bacillus thuringiensis , bacillales , bacillus (shape) , chemistry , biology , hydrolysis , bacteria , biochemistry , genetics , organic chemistry
Incubation of sporulating cultures of Bacillus anthracis, B. cereus, B. subtilis and B. thuringiensis in 1°0 mol/l sodium sulphate markedly increased the release of free spores from sporangia. It is postulated that the release of spores is due to activation of latent autolysins which hydrolyse sporangial cell walls. Sodium sulphate‐induced lysis of sporangia represents a novel and highly effective method for the recovery of spores from cultures of Bacillus species.