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Molecular relationships between trimethoprim R plasmids obtained from human and animal sources
Author(s) -
Towner K. J.,
Wise P. J.,
Lewis M. J.
Publication year - 1986
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1986.tb01726.x
Subject(s) - plasmid , restriction enzyme , biology , escherichia coli , genetics , endonuclease , human animal , microbiology and biotechnology , dna , gene , domestication
A total of 65 trimethoprim R plasmids (35 obtained from human strains and 30 from animal strains of Escherichia coli ) were examined by the technique of restriction endonuclease fingerprinting. Plasmids belonging to incompatibility groups B, F II and 1Δ obtained from human and animal sources showed close similarities within each group. Plasmids belonging to incompatibility groups 1α and P were also obtained from both human and animal sources, but there was no conclusive evidence of close relationships within the groups. Restriction endonuclease fingerprinting was found to be useful for obtaining information about the epidemiology of R plasmids. Its main limitation in this study related to broad host range plasmids of the P incompatibility group, some of which contained very few sites susceptible to cleavage by the restriction endonucleases tested.