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Scanning electron microscopy preparation methods: their influence on the morphology and fibril formation in Pseudomonas fragi (ATCC 4973)
Author(s) -
Fraser T. W.,
Gilmour A.
Publication year - 1986
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1986.tb01092.x
Subject(s) - scanning electron microscope , morphology (biology) , fibril , electron microscope , chemistry , dehydration , materials science , chemical engineering , biophysics , biochemistry , biology , composite material , genetics , physics , optics , engineering
The use of cryo‐scanning electron microscopy (cryo‐SEM) in the study of the morphology of Pseudomonas fragi (ATCC 4973) revealed gross differences when compared to material prepared using conventional chemical methods. Changes associated with each step of the chemical preparatory procedure were monitored by cryo‐SEM. It appeared that fibril formation was associated with the ethanol dehydration stage of the chemical preparation method and was not an attachment feature of these cells.