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Estimation of Gram‐negative bacteria in milk: A comparison of inhibitor systems for preventing Gram‐positive bacterial growth
Author(s) -
Phillips J. D.,
Griffiths M. W.
Publication year - 1986
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1986.tb01088.x
Subject(s) - gram , crystal violet , gram negative bacteria , bacteria , microbiology and biotechnology , gram positive bacteria , nisin , biology , monensin , chemistry , food science , antibiotics , escherichia coli , biochemistry , antimicrobial , genetics , gene
A number of inhibitor systems which have been reported to allow selection of Gram‐negative bacteria were tested against Gram‐positive and Gram‐negative isolates of dairy origin. No one system worked perfectly. A mixture of crystal violet‐penicillin‐nisin or monensin had least inhibitory effect on Gram‐negative isolates whereas Selectocult (a commercially available mixture of Benzalkon A 50% and crystal violet) and sodium deoxycholate were the most effective inhibitors of Gram‐positive bacteria. Cetrimide‐fucidin‐cephaloridine solutions, which have been reported as allowing selective growth of pseudomonads, were not so specific when applied to milk systems.