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Nitrogen metabolism in Aspergillus parasiticus NRRL 3240 and A. flavus NRRL 3537 in relation to aflatoxin production
Author(s) -
Bhatnagar R. K.,
Ahmad S.,
Mukerji K. G.,
Venkitasubramanian T. A.
Publication year - 1986
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1986.tb01074.x
Subject(s) - glutamate synthase , glutamate dehydrogenase , biochemistry , aspergillus flavus , glutamine synthetase , aflatoxin , glutamate receptor , metabolism , nad+ kinase , transaminase , biology , glutamine , aspergillus parasiticus , chemistry , enzyme , microbiology and biotechnology , food science , amino acid , receptor
The relationship between nitrogen assimilation, metabolism and aflatoxin formation has been investigated in a toxigenic and a non‐toxigenic strain of Aspergillus parasiticus. Ammonia from the medium is mainly assimilated via NADP‐requiring glutamate dehydrogenase. During growth NAD‐requiring glutamate dehydrogenase followed an inverse pattern of activity with respect to NADP glutamate dehydrogenase. Alpha‐ketoglutarate, the product of NAD glutamate dehydrogenase, stimulated acetate incorporation into aflatoxins. Glutamine synthetase, ornithine transcarbamylase, both utilizing glutamate as substrate were assayed under different growth conditions. An important regulatory role for glutamine synthetase is suggested. The metabolic route of asparagine utilization was also investigated. Both the known pathways, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase are operative simultaneously.