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Thermal resistance variations due to post‐harvest treatments in Bacillus subtilis spores
Author(s) -
Gorman S.P.,
Scott E.M.,
Hutchinson E.P.
Publication year - 1985
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1985.tb03360.x
Subject(s) - spore , bacillus subtilis , dipicolinic acid , dithiothreitol , urea , calcium , chemistry , food science , microbiology and biotechnology , biochemistry , biology , bacteria , enzyme , organic chemistry , genetics
Spores of Bacillus subtilis NCTC 10073 were produced using three different methods on solid and liquid defined media. Thermal resistance at 90d̀C was examined in relation to the effects of cleaning procedures such as number of washings, speed and temperature of centrifugation on the spores. Ion‐exchange spore forms (hydrogen and calcium) and coat‐defective forms (urea‐mercaptoethanol and urea‐dithiothreitol‐sodium dodecyl sulphate) were prepared from spores produced by the different spore production methods and compared for thermal resistance. The resistance patterns observed are discussed in terms of the spore properties of germinability. colony formation, dipicolinic acid and calcium contents.