Premium
High‐performance liquid chromatographic identification of beta‐lactam antibiotics produced by dermatophytes
Author(s) -
LappinScott H.M.,
Rogers M.E.,
Adlard M.W.,
Holt G.,
Noble W.C.
Publication year - 1985
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1985.tb03343.x
Subject(s) - penicillin , microsporum canis , chromatography , antibiotics , microbiology and biotechnology , high performance liquid chromatography , dermatophyte , chemistry , derivatization , bioassay , trichophyton , fermentation , phenylacetic acid , biology , biochemistry , antifungal , genetics
Thirteen of 48 dermatophyte isolates were found by bioassay to produce beta‐lactam antibiotics and seven produced other antibiotics. Estimation and detection of specific beta‐lactams in culture broths by derivatization and HPLC was only possible following concentration and extraction procedures. Analysis of the concentrated broths demonstrated the production of penicillin X and penicillin G by two Trichophyton mentagrophytes strains and by one Microsporum canis strain; one further T. mentagrophytes strain produced only penicillin X. Additions of the beta‐lactam side‐chain precursors, phenylacetic acid and phenoxyacetic acid, to fermentation media failed to increase the antibiotic titres.