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Biotransformation of sugar‐cane sterols into Androsta 1,4‐dione‐3,17‐dione (ADD) by Arthrobacter globiformis Str. Oxydans
Author(s) -
Srivastava S.K.,
Srivastava R.A.K.,
Mathur S.N.
Publication year - 1985
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1985.tb03338.x
Subject(s) - bioconversion , chemistry , biotransformation , ketosteroid , arthrobacter , steroid , sugar , sucrose , chromatography , sodium , biochemistry , organic chemistry , hormone , fermentation , isomerase , gene , enzyme
Arthrobacter globiformis isolated from garden soil was cultured on phosphate salt medium containing phytosterols extracted from sulphitation pressmud, a sugar industry waste, as sole source of carbon. The bacterium grew well on sterols and transformed them into precursors of steroidal drugs and hormones. Addition of α,α′‐dipyridyl and sodium arsenite as metabolic inhibitors in the culture medium enhanced the accumulation of a 17‐ketosteroid which acts as precursor of steroidal drugs. The optimum temperature and pH conditions for its maximum accumulation were found to be 32 ± 0.5d̀C and 7.2. Spectroscopic analyses by u.v., i.r., p.m.r. and mass spectra of the 17‐ketosteroid formed by microbial activity in the medium confirmed it to be androsta‐1,4‐diene‐3,17‐dione (ADD). The bioconversion of sugar‐cane sterols into 17‐ketosteroid in varying culture condition is discussed.