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Identification of fusobacteria in a routine diagnostic laboratory
Author(s) -
Bennett K.W.,
Duerden B.I.
Publication year - 1985
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1985.tb03318.x
Subject(s) - fusobacteria , microbiology and biotechnology , fusobacterium , biology , lecithinase , bacteroides , 16s ribosomal rna , bacteria , bacteroidetes , genetics
A scheme for differentiating Fusobacterium spp. and Leptotrichia spp. from Bac‐teroides spp, was devised after examining 114 strains of fusobacteria and asac‐charolytic bacteroides (17 reference strains and 97 clinical isolates). Sensitivity to a 300 μg/ml plate of phosphomycin and an acid reaction on a lysine plate were found to be reliable for differentiating Fusobacterium spp. and L. buccalis from Bacteroides Using a short set of simple cultural and biochemical tests, isolates could be identified as F. necrophorum, F. necrogenes, F. nucleatum, F. varium or L. buccalis . These tests were: indole, lecithinase, phosphatase, DNase and gas production, aesculin and casein hydrolysis, greening of casein/methylene blue agar, nitrite reduction, bile tolerance and haemolysis on horse blood agar.