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Mutagenesis of Clostridium acetobutylicum
Author(s) -
BOWRING SARAH N.,
MORRIS J.G.
Publication year - 1985
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1985.tb01714.x
Subject(s) - clostridium acetobutylicum , clostridia , mutagenesis , auxotrophy , clostridium , clostridium beijerinckii , mutant , microbiology and biotechnology , chemistry , obligate anaerobe , bacteria , biology , biochemistry , gene , genetics , butanol , ethanol
Mutagenesis of the obligate anaerobe Clostridium acetobutylicum was best accomplished using agents (e.g. ethyl methane sulphonate or N ‐methyl‐ N '‐nitro‐ N ‐nitrosoguanidine) which are believed to act by a direct mutagenic mechanism. Other agents (e.g. u.v. radiation) whose effectiveness relies on misrepair of damaged DNA via an error‐prone pathway, were poor mutagens of this organism. Procedures are described which readily yielded a variety of auxotrophic and other useful mutant strains of Cl. acetobutylicum and related saccharolytic clostridia.