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Analysis of cellular fatty acids by gas chromatography as a tool in the identification of medically important coryneform bacteria
Author(s) -
ATHALYE M.,
NOBLE W.C.,
MINNIKIN D.E.
Publication year - 1985
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1985.tb01491.x
Subject(s) - corynebacterium diphtheriae , bacteria , fatty acid , gas chromatography , corynebacterium , chemistry , microbiology and biotechnology , biology , pathogenic bacteria , biochemistry , chromatography , diphtheria , virology , genetics , vaccination
The fatty acid methyl esters of nineteen unidentified pathogenic coryneform bacteria were analysed by gas‐liquid chromatography and the resulting profiles were compared with those of type or reference strains of possibly related species, namely Caseobacter polymorphus, Corynebacterium bovis, C. diphtheriae, C. xerosis and Rhodococcus equi. All of the strains had distinct fatty acid profiles but most of them conformed to a general pattern, with high levels of octadecanoic acids and only trace amounts of 10‐methyl octadecanoic acid (tuberculostearic acid). These profiles were very similar to those from C. diphtheriae and C. xerosis but could be differentiated from C. bovis, Cas. polymorphus, R. equi and two unidentified pathogenic strains which had significantly higher levels of tuberculostearic acid.