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Determination of susceptibility of Staphylococcus aureus to methicillin by luciferim‐luciferase assay of bacterial adenosine triphosphate
Author(s) -
McWalter P.W.
Publication year - 1984
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1984.tb04706.x
Subject(s) - luciferase , staphylococcus aureus , adenosine triphosphate , microbiology and biotechnology , chemistry , adenosine , bacteria , biochemistry , biology , transfection , genetics , gene
M c W alter , P.W. 1984. Determination of susceptibility of Staphylococcus aureus to methicillin by luciferin‐luciferase assay of bacterial adenosine triphosphate. Journal of Applied Bacteriology , 56 , 145–150. Susceptibility of 50 strains of Staphylococcus aureus to methicillin was determined by disc diffusion, minimum inhibitory concentration (MIC) test and luciferin‐luciferase assay of bacterial adenosine triphosphate (ATP). ATP concentration time curves calculated on eight strains (four sensitive, four resistant) incubated at 30d̀C indicated 2.5 h as the optimum time for determination of methicillin susceptibility. The ATP concentration and relative light unit (RLU) value of each test broth (antibiotic‐containing), expressed as a percentage of its own control broth (antibiotic‐free) indicated values of < 30% for ATP and < 40% for RLU to be indicative of methicillin sensitivity. Single time point ATP assays carried out on the 50 strains after 2.5 h at 30d̀C correlated exactly with disc diffusion and MIC. Luciferin‐luciferase assay of bacterial ATP appeared to be a reliable, rapid technique for determining the susceptibility of Staph. aureus to methicillin.