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Hydrogen‐using bacteria in a methanogenic acetate enrichment culture
Author(s) -
Archer D.B.
Publication year - 1984
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1984.tb04703.x
Subject(s) - bacteria , enrichment culture , microbiology and biotechnology , microbiological culture , chemistry , anaerobic bacteria , food science , biology , genetics
A rcher , D.B. 1984. Hydrogen‐using bacteria in a methanogenic acetate enrichment culture. Journal of Applied Bacteriology 56 , 125–129. In a study of the anaerobic utilization of acetate, an enrichment culture of sewage sludge organisms was initiated with calcium acetate as the sole carbon and energy source. A mixed bacterial population became established from which 14 anaerobic species were isolated. Two of the isolates were methanogenic bacteria but only one of these, Methanosarcina barkeri , utilised acetate as an energy source in axenic culture. The other methanogenic isolate, a Methanobacterium sp., utilised H 2 /CO 2 but not acetate. A third methanogen, which was morphologically identical to Methanothrix soehngenii , was detected in the enrichment but was not obtained in monoculture. 2‐Bromoethanesulphonate, a specific inhibitor of methanogenesis. completely inhibited the enrichment at a concentration of 10 μmol/1. Addition of H 2 formate or methanol to the enrichment did not affect the rate of methanogenesis. An H 2 ‐utilizing Desulfovibrio sp. was also isolated from the enrichment.