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The enumeration of Bacteroides fragilis group organisms from sewage and natural waters
Author(s) -
Allsop K.,
Stickler D.J.
Publication year - 1984
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1984.tb04692.x
Subject(s) - enumeration , bacteroides fragilis , sewage , microbiology and biotechnology , bacteroides , biology , natural (archaeology) , group (periodic table) , environmental science , chemistry , bacteria , mathematics , combinatorics , environmental engineering , antibiotics , genetics , paleontology , organic chemistry
A llsop K. & S tickler D. J. 1984. The enumeration of Bacteroides fragilis group organisms from sewage and natural waters. Journal of Applied Bacteriology 56 , 15–24. A membrane filtration technique has been developed for the enumeration of Bacteroides fragilis group (BFG) organisms from sewage and natural waters. The method uses the agar medium of Wilkins and Chalgren supplemented with gentamicin. penicillin, aesculin and ferric ammonium citrate. Membrane filters with 0.22 μm pores were significantly more efficient than those with 0.45 μm pores in the isolation of BFG. A preliminary incubation period of 4 h at 30d̀C prior to 44 h at 37d̀C yielded significantly higher numbers of BFG than direct incubation at 37d̀C for 48 h.