Premium
Regulation of amylase bio‐synthesis in growing and non‐growing cells of Bacillus stearothermophilus
Author(s) -
Srivastava R.A.K.,
Mathur S.N.
Publication year - 1984
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1984.tb02367.x
Subject(s) - amylase , maltose , biochemistry , chloramphenicol , starch , gtp' , rna polymerase , rna , enzyme , protein biosynthesis , biology , de novo synthesis , chemistry , antibiotics , gene
The regulation of amylase synthesis in growing and non‐growing cells of a thermophilic Bacillus stearothermophilus was studied. Starch induced its synthesis in growing cells, while glucose and maltose repressed it. Glucose, however, had no effect on amylase synthesis in non‐growing cells for the first 3 h. Cyclic‐AMP acted as a derepressor and completely reversed the glucose effect in growing cultures when added during the lag or the exponential phases. Apart from ATP, other nucleotides such as AMP, ADP, GMP, GDP and GTP, had no effect on amylase synthesis. The inorganic phosphates (K 2 HPO 4 , Na 2 HPO 4 ) up to about 240 mmol affected amylase synthesis/secretion in growing and non‐growing conditions, whereas the organic phosphates (glucose‐6‐P and glucose‐1‐P) did not. The addition of RNA polymerase inhibitors such as actinomycin D (1 μg/ml), and rifampicin (1 μg/ml) stopped amylase synthesis by washed cells after 3 h whereas the protein synthesis inhibitor, chloramphenicol (20 μg/ml) stopped amylase synthesis instantaneously. This indicated that the m‐RNA for amylase synthesis was long lived and therefore synthesis continued even in the presence of RNA polymerase inhibitors. The glucose effect observed after 3 h was identical to that of the RNA polymerase inhibitors which indicated that glucose repression of amylase synthesis was probably at the transcriptional level.