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The modification and evaluation of some cytochemical techniques for the enumeration of metabolically active heterotrophic bacteria in the aquatic environment
Author(s) -
Quinn J.P.
Publication year - 1984
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1984.tb02355.x
Subject(s) - nalidixic acid , enumeration , bacteria , biology , benthic zone , substrate (aquarium) , heterotroph , microbiology and biotechnology , food science , chemistry , environmental chemistry , ecology , mathematics , genetics , antibiotic resistance , combinatorics
Variants of the tetrazolium‐reduction, nalidixic acid‐inhibition and fluorescein diacetate‐hydrolysis techniques for enumeration of metabolically active bacteria were compared, using samples of planktonic, benthic, and epiphytic freshwater bacteria. Results obtained by these methods generally showed statistically significant differences. However, an INT reduction technique, without added substrate, and a slightly modified nalidixic acid procedure gave values which did not differ at the 5% level. The results indicated that from 10 to 40% of total bacteria in the samples examined were metabolically active. These values were up to two orders of magnitude higher than those obtained by conventional plate count techniques.