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Enumeration of two competing Erwinia carotovora populations in potato tubers by a membrane filter‐immunofluorescence procedure
Author(s) -
Boer S.H. De
Publication year - 1984
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1984.tb01418.x
Subject(s) - erwinia , inoculation , strain (injury) , membrane filter , population , biology , incubation , immunofluorescence , microbiology and biotechnology , indirect immunofluorescence , solanaceae , horticulture , botany , bacteria , membrane , genetics , gene , biochemistry , antibody , anatomy , demography , sociology
Relative increases in cell populations of specific Erwinia carotovora strains injected into potato tubers either singly or in combination of two strains were determined by depositing tissue extracts on membrane filters and staining cells of individual strains by immunofluorescence. The population increase of an Erw. carotovora subsp. atroseptica (Eca) strain was, in general, not affected by the presence of an Erw. carotovora subsp. carotovora (Ecc) strain. However, the increase of an Ecc strain was inhibited by the presence of an Eca strain, especially at incubation temperatures of 5° and 15°C but not at 26°C. One Ecc strain consistently increased in population over another Ecc strain at a greater rate when they were inoculated together at the same loci in comparison to when they were inoculated separately at different loci.