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An improved enzyme‐linked immunoassay for the detection and quantification of the entomocidal parasporal crystal proteins of Bacillus thuringiensis subspp. kurstaki and israelensis
Author(s) -
Wie Siong I.,
Hammock B.D.,
Gill Sarjeet S.,
Grate Elizabeth,
Jr. R.E. Andrews,
Faust R.M.,
Jr. L.A. Bulla,
Schaefer C.H.
Publication year - 1984
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1984.tb01410.x
Subject(s) - bacillus thuringiensis , glutaraldehyde , bacillaceae , immunoassay , cuvette , bacillales , chromatography , enzyme , microbiology and biotechnology , chemistry , antibody , biology , bacteria , biochemistry , immunology , physics , genetics , quantum mechanics , bacillus subtilis
An improved and simplified enzyme‐linked immunosorbent assay (ELISA) was developed for the detection and quantification of parasporal crystalline toxins from Bacillus thuringiensis subsp. kurstaki . The improved procedure involved pretreatment of the polystyrene cuvettes with glutaraldehyde before antibody coating. A direct comparison of treated and untreated cuvettes is provided. ELISAs were then used for the analysis of the entomocidal crystalline proteins in commercial and experimental formulations of B. thuringiensis subspp. kurstaki and israelensis .