Premium
Isolation of Yersinia enterocolitica and related species from porcine samples obtained from an abattoir
Author(s) -
Harmon M.C.,
Swaminathan B.,
Forrest J.C.
Publication year - 1984
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1984.tb01370.x
Subject(s) - yersinia enterocolitica , rappaport , bacteriology , microbiology and biotechnology , sorbitol , biology , isolation (microbiology) , agglutination (biology) , phosphate buffered saline , chemistry , bacteria , chromatography , food science , antibody , philosophy , genetics , theology , immunology
H armon , M.C., S waminathan , B. & F orrest , J.C. 1984. Isolation of Yersinia enterocolitica and related species from porcine samples obtained from an abattoir. Journal of Applied Bacteriology 56 , 421–427. Swabs of swine carcasses and samples of porcine tongue and trim obtained from an abattoir were examined for the presence of Yersinia enterocolitica and related species ( Y. intermedia, Y. kristensenii and Y. frederiksenii) . Three enrichment media (phosphate buffered saline, sorbitol‐bile salts‐phosphate buffered saline, and a modified Rappaport's broth) were compared at 4d̀C for their efficiency of recovery of Y. enterocolitica and related species. Two secondary enrichment procedures (post‐enrichment in modified Rappaport's broth for 2 d at 25d̀C and treatment with 0.5% KOH in 0.5% NaCl) also were evaluated. The porcine isolates were characterised by biochemical and serological examination, speciation, and biotyping. Eight of 43 samples were positive for Y. enterocolitica and related species. The combination of incubation in sorbitol‐bile salts‐phosphate buffered saline for 21 d at 4d̀C followed by post‐enrichment in modified Rappaport's broth yielded maximum number of isolates. All isolates, except one, were avirulent as determined by auto‐agglutination, calcium dependence at 37d̀C, and HeLa cell invasiveness tests.