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Comparative study on Salmonella isolation from sewage—contaminated natural waters
Author(s) -
Alcaide Elena,
Martinez J.P.,
Garay Esperanza
Publication year - 1984
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1984.tb01363.x
Subject(s) - salmonella , agar , isolation (microbiology) , sewage , contamination , brilliant green , plating (geology) , food science , serotype , chemistry , microbiology and biotechnology , biology , veterinary medicine , environmental science , environmental engineering , bacteria , medicine , ecology , paleontology , genetics , organic chemistry
A lcaide , E., M artinez , J.P. & G aray , E. 1984. Comparative study on Salmonella isolation from sewage contaminated natural waters. Journal of Applied Bacteriology 56 , 365–371. A comparative study of five factors influencing the isolation of salmonellas from sewage‐contaminated natural waters was carried out. The effect of pre‐enrichment in buffered peptone water was compared with single‐step enrichment in NR10 broth incubated at 43d̀C. A modification of NR10 has been compared with the original composition. Bismuth sulphite agar (BSA), Hektoen enteric agar (HE) and brilliant green agar (BGA) have been used as plating media. Other factors considered have been temperature of the water and sampling site. A total of 759 salmonella strains belonging to 36 different serotypes has been recovered in a two‐year study. All five factors considered in the study have shown a significant effect on the recovery of salmonellas. The combination of direct enrichment in NR10, followed by BSA or HE as plating media was most effective for the isolation of Salmonella . The influence of water temperature and characteristics of the sampling sites have also been discussed.