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Detection of chemotaxis in Azospirillum brasilense
Author(s) -
Barak Rina,
Nur I.,
Okon Y.
Publication year - 1983
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1983.tb02634.x
Subject(s) - chemotaxis , azospirillum brasilense , agar , agar plate , bacteria , strain (injury) , microbial inoculant , chemistry , amino acid , biochemistry , microbiology and biotechnology , biology , anatomy , receptor , genetics
Azospirillum brasilense strain Cd responded chemotactically to amino acids, sugars and organic acids. Chemotactic rings were observed in semisolid agar plates containing oxidizable substrates. Increasing sodium succinate concentration decreased the velocity of ring expansion. Chemotactic activity of Azospirillum was also examined by a newly developed technique using a channelled chamber. Varying the concentrations of aspartic and glutamic acids affected the chemotactic response of the bacteria. In both assays chemotaxis was obtained under conditions that prevented aerotaxis.