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Rapid method for selecting appropriate solid media for the enumeration of aerobic micro‐organisms
Author(s) -
Richard J.,
Andersen Hanne Møller,
Gratadoux J.J.
Publication year - 1983
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1983.tb02624.x
Subject(s) - enumeration , petri dish , spiral (railway) , computer science , mathematics , biology , microbiology and biotechnology , combinatorics , mathematical analysis
A quick and cheap method for selecting appropriate solid culture media has been devised. It consists in the rapid picking of fragments of test colonies with the aid of a rubber strip in which pins are fixed in parallel, dispensing up to 8 colonies simultaneously in the wells of a Microtiter plate and streaking 4 strains at the same time on square Petri dishes containing the media under comparison. The approximate diameters of well‐isolated colonies are measured with the aid of a series of calibrated spots. The results corresponded with those given by the spiral plate method used as a reference for colony count and diameter measure.