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Chemostat adaptation of Escherichia coli B/r/1 to low water activity
Author(s) -
Anagnostopoulos G.D.,
Dhavises G.
Publication year - 1982
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1982.tb04674.x
Subject(s) - chemostat , dilution , escherichia coli , chemistry , yield (engineering) , food science , growth rate , microbiology and biotechnology , biology , biochemistry , bacteria , mathematics , materials science , thermodynamics , gene , physics , genetics , geometry , metallurgy
Chemostat cultures of Escherichia coli B/r/1 under conditions of glucose limitation in a salts medium at high water activity ( a w of 0.999) and at an a w of 0.987, controlled by NaCl, have been compared. The system was run at dilution rates above 0.035 h ‐1 . The results suggested that the organism adapted to the lower a w of the medium as no significant change was observed in the energy requirement for maintenance, although the maximum molar growth yield for glucose decreased by 23.2%. Such cultures showed also a shorter lag and a higher growth rate in batch at an a w of 0.987, as compared with cultures initiated with an unadapted ( a w of 0.999) inoculum.