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A note on the performance of Rappaport's medium, compared with Rappaport‐Vassiliadis broth, in the isolation of salmonellas from meat products, after pre‐enrichment
Author(s) -
Xirouchaki E.,
Vassiliadis P.,
Trichopoulos D.,
Mavrommati CH.
Publication year - 1982
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1982.tb04384.x
Subject(s) - rappaport , malachite green , tetrathionate , incubation , isolation (microbiology) , chemistry , food science , microbiology and biotechnology , biology , biochemistry , philosophy , theology , organic chemistry , adsorption , thiosulfate , sulfur
Salmonellas were isolated from meat products using a slightly modified Rappaport's enrichment medium (R25), Rappaport‐Vassiliadis procedure (Rappaport's broth containing 10 ml instead of 30 ml of Malachite Green solution and incubated at 43 o C instead of 37 o C), and Muller‐Kauffmann's tetrathionate broth. From 255 samples, 89 were found positive with the Rappaport‐Vassiliadis procedure, 83 with the R25 broth, whereas only 43 were positive with Muller‐Kauffmann broth. It is concluded that the R25 medium may be used as an alternative to the more effective Rappaport‐Vassiliadis broth when the only available incubation temperature is 37 o C.