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Rapid Counting of Bacteria in Rinses of Milking Equipment by a Membrane Filtration ‐ Epifluorescent Microscopy Technique
Author(s) -
McKIN C. H.,
MANSELL R.
Publication year - 1981
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1981.tb01253.x
Subject(s) - bacteria , milking , acridine orange , filtration (mathematics) , chromatography , hemocytometer , pipette , video microscopy , membrane , chemistry , membrane filter , microbiology and biotechnology , biology , biochemistry , mathematics , zoology , apoptosis , genetics , statistics
Membrane filtration and epifluorescent microscopy were used for the rapid enumeration of bacteria in rinses of milking equipment. Rinse solution (10 ml) was filtered through a 0.6 µm pore size Nuclepore membrane filter and the bacteria retained stained with acridine orange. The clump count of orange fluorescing bacteria on the membrane correlated well with the corresponding plate count ( r =0.83). The technique is rapid, taking approximately 10 min, and is sufficiently sensitive to detect 1000 bacteria ml rinse (equivalent to 5 × 10 3 bacteria/m 2 of milking equipment surfaces by the method currently in use).