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Potassium Fluxes on Hyperosmotic Shock and the Effect of Phenol and Bronopol (2‐bromo‐2‐nitropropan‐1,3‐diol) on Deplasmolysis of Pseudomonas aeruginosa
Author(s) -
KROLL R. G.,
ANAGNOSTOPOULOS G. D.
Publication year - 1981
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1981.tb01248.x
Subject(s) - glycerol , sucrose , chemistry , potassium , proline , phenol , penetration (warfare) , mannitol , chromatography , osmotic concentration , phenol red , biochemistry , organic chemistry , amino acid , operations research , engineering
Potassium fluxes and the effect of phenol and bronopol on deplasmolysis of Pseudomonas aeruginosa were followed in sucrose and glycerol plasmolysing systems. In sucrose, K + uptake related to the solute concentration. Proline increased the rate and overall K + uptake, the latter by a factor of three. It was concluded that there was no rigid maximum in the accumulation of intracellular K + as long as intracellular neutrality in electrical charges was maintained. In glycerol, K + uptake was parallel with glycerol penetration. The process was reversed, however, on equilibration of glycerol. This suggested that glycerol inhibited K + retention against a concentration gradient rather than that K + was excluded as a consequence of the osmotic established steady state. This view was enforced by the fact that the reversal of K + uptake occurred in 20 and 30% glycerol but not in 10%. Phenol and bronopol did not affect deplasmolysis in glycerol significantly, although some effect on K + uptake and glycerol permeability could be seen. In the sucrose system, phenol acted according to its mode of action generally accepted, i.e. inhibiting deplasmolysis at low and allowing solute penetration at higher concentrations, whereas very high concentrations caused coagulation of the cytoplasm. Bronopol inhibited deplasmolysis, except at very low concentrations. Proline did not prevent the inhibition of deplasmolysis in either of the solute systems, except at the very low bronopol concentrations where the deplasmolysis rate only was affected.