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Numerical Taxonomy Analysis of Bacteria Isolated from the Completed ‘Most Probable Numbers’ Test for Coliform Bacilli
Author(s) -
AUSTIN B.,
HUSSONG D.,
WEINER R. M.,
COLWELL R. R.
Publication year - 1981
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1981.tb00913.x
Subject(s) - biology , microbiology and biotechnology , citrobacter , bacilli , serratia , citrobacter freundii , acinetobacter calcoaceticus , bacteria , enterobacteriaceae , oyster , acinetobacter , providencia , proteus mirabilis , escherichia coli , pseudomonas , antibiotics , ecology , staphylococcus aureus , biochemistry , genetics , gene
Lactose‐fermenting bacteria were isolated from oyster, water and sediment samples tested to determine the Most Probable Number (MPN) of coliforms present. The completed test series was used to enumerate the total number of coliforms. The strains from the completed tests of the MPN analysis were compared with named reference cultures, for which 100 phenotypic characters were recorded and the taxonomic data analysed by methods of numerical taxonomy. The isolates were recovered in 13 phenetic groups, defined at or above the 80% similarity level. Nine phena were identified as Acinetobacter calcoaceticus, Chromobacterium violaceum, Citrobacter freundii, Erwinia herbicola, Escherichia coli, Hafnia alvei, Klebsiella pneumoniae, Serratia liquefaciens , and Ser. marcescens. Of these, Ac. calcoaceticus and C. violaceum were isolated exclusively from oyster tissue samples and strains of the other taxa from both water and oyster samples. Some strains of Esch. coli, Er. herbicola, H. alvei and bacteria that were not Enterobacteriaceae were found to lose the ability to ferment lactose, following storage on laboratory media. Identifications from diagnostic keys, rapid identification systems and IMViC patterns were not always in agreement.