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Storage of Escherichia coli Strains Containing Plasmid DNA in Liquid Nitrogen
Author(s) -
BREESE M. D.,
SHARP R. J.
Publication year - 1980
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1980.tb05207.x
Subject(s) - plasmid , escherichia coli , lysogenic cycle , plasmid preparation , microbiology and biotechnology , dna , bacteriophage , bacteria , liquid nitrogen , chemistry , biology , genetics , gene , pbr322 , organic chemistry
The stability of plasmids and lysogenic bacteriophage in 16 strains of Escherichia coli was studied following storage in liquid nitrogen for periods up to 2 years. A comparison was made with cultures which had undergone lyophilization. Plasmid loss was detected by plating on selective media containing antibiotics to which resistance was conferred by plasmid DNA. Loss of plasmid DNA was not evident following storage in liquid nitrogen or lyophilization.