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A Note on the Development of a Technique for Regeneration of a Degenerated Culture
Author(s) -
KOLE H. K.,
BOSE S. K.
Publication year - 1980
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1980.tb01032.x
Subject(s) - regeneration (biology) , organism , biology , agar , streak , tissue culture , streaking , agar plate , microbiology and biotechnology , genetics , bacteria , in vitro , chemistry , mineralogy
In a heterogeneous degenerated culture the method of in situ screening of established agar colonies (the number screened being limited) cannot reveal the presence of an antibiotic‐producing organism even after mutagenic treatment, whereas the close‐range parallel streak method can succeed. Streaking a pour‐plate culture of an antibiotic‐sensitive organism by parallel streaks of mutagen‐treated inactive culture of the once producer afforded a better chance for regeneration of a degenerated culture.