Premium
The Effect of Hydrogen Peroxide and Ultraviolet Irradiation on Non‐sporing Bacteria
Author(s) -
BAYLISS CATHERINE E.,
WAITES W. M.
Publication year - 1980
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1980.tb01030.x
Subject(s) - hydrogen peroxide , incubation , irradiation , escherichia coli , bacteria , chemistry , microbiology and biotechnology , ultraviolet , ultraviolet irradiation , peroxide , ultraviolet light , hydrogen , photochemistry , materials science , biology , biochemistry , organic chemistry , physics , genetics , optoelectronics , nuclear physics , gene
A kill of 99.99% was obtained in cell suspensions of Escherichia coli and Streptococcus faecalis by incubation with hydrogen peroxide 1.0% (w/v) for 75 and 180 min respectively. The same kill was produced by 30 s irradiation with ultraviolet (u.v.) light in the presence of hydrogen peroxide 1.0% (w/v). This simultaneous treatment with u.v. and hydrogen peroxide produced a synergistic kill at least 30‐fold greater than that produced by irradiation of cell suspensions of Esch. coli with or without subsequent incubation with hydrogen peroxide.