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A Note on the Enumeration of Epiphytic Bacteria by Microscopic Methods with Particular Reference to Two Freshwater Plants
Author(s) -
HOSSELL JUDITH C.,
BAKER J. H.
Publication year - 1979
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1979.tb02584.x
Subject(s) - bacteria , staining , biology , botany , epiphyte , population , fluorescence microscope , fluorescent staining , eosin , chemistry , fluorescence , genetics , demography , physics , quantum mechanics , sociology
Bacteria on plant surfaces were examined using epifluorescence, bright‐field microscopy and an impression technique. Staining bacteria directly on the plant surface with phenolic aniline blue was found to be the best method to use for the determination of bacterial density. The effect on the estimation of population density of pretreatment of the plant with agents such as methanol and eosin yellowish was investigated. The average sizes of the bacterial populations on two freshwater plants, Rorippa and Lemna , estimated after staining by this method, were 5 times 10 6 and 9 times 10 6 bacteria cm‐ z respectively.