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Fluorigenic Glycosidase Substrates: Their Use in the Identification of some slow growing Mycobacteria
Author(s) -
GRANGE J. M.,
McINTYRE G.
Publication year - 1979
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1979.tb01755.x
Subject(s) - glycoside hydrolase , mycobacterium marinum , mycobacterium , microbiology and biotechnology , biology , identification (biology) , enzyme , biochemistry , bacteria , botany , genetics
Glycosidase activity in slow growing mycobacteria was rapidly detected by the use of 4‐methylumbelliferone‐linked glycoside substrates. The species Mycobacterium nonchromogenicum, M. szulgai and M. marinum showed characteristic enzyme activities which distinguished them from the other species. M. marinum is unique among the mycobacteria so far examined in its possession of a very marked α‐L‐fucosidase activity. These fluorigenic glycosidase substrates should be of value in the identification of slow growing mycobacteria.

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