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The Combined Effect of Hydrogen Peroxide and Ultraviolet Irradiation on Bacterial Spores
Author(s) -
BAYLISS CATHERINE E.,
WAITES W. M.
Publication year - 1979
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1979.tb01753.x
Subject(s) - hydrogen peroxide , spore , bacillus subtilis , irradiation , chemistry , microbiology and biotechnology , peroxide , endospore , ultraviolet irradiation , clostridium , ultraviolet light , bacillus (shape) , bacteria , photochemistry , biology , biochemistry , organic chemistry , genetics , physics , nuclear physics
Ultra‐violet (u.v.) light irradiation of spores of Bacillus subtilis in the presence of hydrogen peroxide produced a rapid kill which was up to 2000‐fold greater than that produced by irradiation alone. A kill of 99–99% was produced by 30s u.v. irradiation of spores of 6 strains of Bacillus and Clostridium in the presence of hydrogen peroxide 1.0 g/100 ml but with the more resistant spores of 9 further strains, irradiation in the presence of hydrogen peroxide 2–5 g/100 ml followed by mild heating was required.