Premium
A Rapid Bioassay Method for Gramicidin by Measuring Rubidium Ion Leakage from Streptococcus faecalis
Author(s) -
MILLER S.J. P.
Publication year - 1979
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1979.tb01180.x
Subject(s) - rubidium , gramicidin , bioassay , enterococcus faecalis , streptococcus , chemistry , gramicidin s , efflux , chromatography , potassium , bacteria , biology , biochemistry , membrane , escherichia coli , organic chemistry , gene , genetics
Gramicidin was seen to promote rapid release of Rb + from cells of Streptococcus faecalis ATCC 10541 which had been previously enriched with this cation. This response was logarithmic between gramicidin concentrations of 0.25 μg/ml and 2.5 μg/ml. This efflux was measured by flame emission spectroscopy. The results presented here form the basis for a potential rapid bioassay for gramicidin.